dc.contributor.advisor | Gómez Garzón, Marcela | |
dc.contributor.advisor | Rodríguez Aristizába, Mónica Alejandra | |
dc.contributor.author | Cufiño Castiblanco, Cristian Sebastián | |
dc.contributor.author | Lara Sanabria, María Alejandra | |
dc.date.accessioned | 2022-10-05T21:31:08Z | |
dc.date.available | 2022-10-05T21:31:08Z | |
dc.date.issued | 2022 | |
dc.identifier.uri | https://repositorio.unicolmayor.edu.co/handle/unicolmayor/5684 | |
dc.description.abstract | El aislamiento y detección de Legionella pneumophila en agua potable puede ser un
proceso difícil, debido a que algunas de las técnicas existentes no concentran ni
recuperan la cantidad estandarizada por entes internacionales, así mismo, es un
microorganismo exigente, y lo tanto, requiere de medios especiales para su
crecimiento. El objetivo del estudio fue validar un método de concentración por medio
de la implementación del equipo Celltrazone para mejorar la recuperación de la
bacteria en agua potable. Para lograr una mejor concentración de L. pneumophila y
que pudiera reflejarse en el medio de cultivo, se ensayaron varias técnicas de
tratamiento para el filtro obtenido por el equipo como el vortex, sonicado y agitación,
sin embargo, el no aplicar ningún procedimiento y realizar una siembra directa desde
la membrana obtuvo los mejores resultados. El análisis estadístico demostró que el
método aplicado puede concentrar hasta 1x102 (100 UFC), se alcanzó una
sensibilidad del 100% y una especificidad del 80% y un valor aceptable de exactitud
(87,5%). El método de filtración de membrana utilizando el equipo Celltrazone permitió
aumentar la concentración del L. pneumophila en agua potable, cumpliendo con los
intervalos normalizados por ISO y la OMS, por lo tanto, el método es apto para su
aplicación permitiendo obtener resultados confiables | spa |
dc.description.abstract | The isolation and detection of Legionella pneumophila in drinking water can be a
difficult process, because some of the existing techniques do not concentrate or
recover the amount standardized by international entities, likewise, it is a demanding
microorganism, and therefore, requires special media for its growth. The objective of
the study was to validate a concentration method through the implementation of
Celltrazone equipment to improve the recovery of the bacterium in drinking water. To
achieve a better concentration of L. pneumophila that could be reflected in the culture
medium, several treatment techniques were tested for the filter obtained by the
equipment, such as vortexing, sonication and agitation; however, not applying any
procedure and performing a direct seeding from the membrane obtained the best
results. Statistical analysis showed that the applied method can concentrate up to
1x102
(100 CFU), a sensitivity of 100% and a specificity of 80% and an acceptable
accuracy value (87.5%) were achieved. The membrane filtration method using the
Celltrazone equipment allowed to increase the concentration of L. pneumophila in
drinking water, complying with the intervals standardized by ISO and WHO, therefore,
the method is suitable for its application allowing to obtain reliable results | eng |
dc.description.tableofcontents | RESUMEN
1. PLANTEAMIENTO DEL PROBLEMA….10
2. HIPÓTESIS11
3. OBJETIVOS..….12
3.1. Objetivo general13
3.2. Objetivos específicos13
4. JUSTIFICACIÓN15
5. ANTECEDENTES… 17
6. MARCO TEÓRICO...21
7. DISEÑO METODOLÓGICO34
7.1. Tipo de investigación...34
7.2. Nivel, alcance o enfoque de la investigación34
7.3. Población objeto de estudio....35
7.4. Muestra35
7.5. Definición de las variables...35
7.6. Procedimientos, técnicas y herramientas para la investigación36
7.7. Validación de los ensayos microbiológicos. 39
7.8. Confirmación en lámina mediante la coloración de Gimenez41
8. RESULTADOS.43
9. DISCUSIÓN..49
10.CONCLUSIONES.....57
11.PERSPECTIVAS PARA EL FUTURO.…58
12.ANEXOS.59
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dc.format.extent | 67p. | spa |
dc.format.mimetype | application/pdf | spa |
dc.language.iso | spa | spa |
dc.rights | Derechos Reservados - Universidad Colegio Mayor de Cundinamarca, 2022 | spa |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-sa/4.0/ | spa |
dc.title | Validación de un método de aislamiento para Legionella pneumophila por medio de técnicas de concentración | spa |
dc.type | Trabajo de grado - Pregrado | spa |
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dc.description.degreename | Abogado(a) | spa |
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dc.rights.accessrights | info:eu-repo/semantics/closedAccess | spa |
dc.rights.creativecommons | Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0) | spa |
dc.subject.lemb | Dilución | |
dc.subject.lemb | Filtración por membrana | |
dc.subject.lemb | Validación | |
dc.subject.lemb | Límite de detección | |
dc.subject.proposal | Legionella pneumophila, | spa |
dc.subject.proposal | Medio BCYE | spa |
dc.subject.proposal | Celltrazone | spa |
dc.type.coar | http://purl.org/coar/resource_type/c_7a1f | spa |
dc.type.coarversion | http://purl.org/coar/version/c_970fb48d4fbd8a85 | spa |
dc.type.content | Text | spa |
dc.type.driver | info:eu-repo/semantics/bachelorThesis | spa |
dc.type.redcol | https://purl.org/redcol/resource_type/TP | spa |
dc.type.version | info:eu-repo/semantics/publishedVersion | spa |
dc.rights.coar | http://purl.org/coar/access_right/c_14cb | spa |