dc.contributor.advisor | Posada Buitrago, Martha Lucía | |
dc.contributor.author | Castillo Usma, Claudia Lorena | |
dc.contributor.author | Guzmán Florez, Andrea Carolina | |
dc.date.accessioned | 2022-03-07T16:35:01Z | |
dc.date.available | 2022-03-07T16:35:01Z | |
dc.date.issued | 2018 | |
dc.identifier.uri | https://repositorio.unicolmayor.edu.co/handle/unicolmayor/4778 | |
dc.description.abstract | La Antártida, continente blanco del hemisferio Austral, cuenta con 14 millones de
km2
, es la reserva de agua dulce más abundante del mundo y punto de equilibrio
de los ecosistemas, presenta factores climáticos extremos para la vida lo que lo
convierte en un campo abierto para la investigación científica.
En este continente, los suelos poseen gran diversidad bacteriana, con escasa
identificación; Por lo anterior el presente trabajo tuvo como objetivo principal
estudiar la diversidad de microorganismos cultivables provenientes de suelos de
la isla Livingston y Decepciòn en la Antártida. La identificación de los organismos
procariotas se logró mediante microbiología tradicional y secuenciación del
producto de amplificación por PCR de la región 16S del rRNA.
Se aislaron 20 microorganismos bacterianos provenientes de estas Islas, de los
cuales se identificaron tres géneros Bacterianos por microbiología convencional:
Salmonella sp., Burkholderia sp., Bacillus sp. y Acinetobacter sp. y un
microorganismo de origen fúngico perteneciente al género Penicillium sp.
Las secuencias obtenidas fueron editadas con el programa Chromas (2.6.5) y
comparadas con el GenBank a través del algoritmo BLASTn. Se identificaron 17
microorganismos del total de aislados, pertenecientes a 11 géneros bacterianos
distribuidos en las dos islas; 8 géneros correspondientes a la Isla Decepción:
Staphylococcus, Oceanisphera, Paeniglutamicibacter, Microbacterium,
Sporosarcina y 6 géneros a la isla Livingston: Burkholderia, Bacillus, y
Acinetobacter, de los géneros identificados tres coincidieron para ambas islas
Brevundimonas, Arthrobacter y Pseudomonas.
La excepcional plasticidad genómica de estos microorganismos, proporciona a la
comunidad científica el potencial para futuros estudios relacionados con
bioprospección. | spa |
dc.description.abstract | Antarctica, white continent of the southern hemisphere, has 14 million km2, is the
reserve of fresh water, most abundant, world and equilibrium point of
ecosystems, has extreme climatic factors for life which makes it an open field for
Scientific research.
In this continent, the soils have great bacterial diversity, with little identification;
With regard to the diversity of microorganisms from the island of Livingston and
Deception in Antarctica. The identification of prokaryotic organisms is carried out
by means of traditional microbiology and sequencing of the PCR amplification
product of the 16S rRNA region.
We isolated 20 bacterial microorganisms from these islands, of which four
Bacterial genera were identified by conventional microbiology: Salmonella sp.,
Burkholderia sp., Bacillus sp. and Acinetobacter sp. And a microorganism of
fungal origin belonging to the genus Penicillium sp.
The sequences were edited with the Chromas program (2.6.5) and compared
with the GenBank through the BLASTn algorithm. Seventeen microorganisms
were identified from the total of the isolates, belonging to 11 bacterial genera
distributed in the two islands; 8 genera corresponding to Deception Island:
Staphylococcus, Oceanisphera, Paeniglutamicibacter, Microbacterium,
Sporosarcina and 6 genera to Livingston Island: Burkholderia, Bacillus, and
Acinetobacter, and the genera of it coincide.
The exceptional genomic plasticity of these microorganisms, the results of
scientific research and the potential for future studies related to bioprospecting | eng |
dc.description.tableofcontents | ÍNDICE DE FIGURAS 10
ÍNDICE DE TABLAS 12
RESUMEN 13
INTRODUCCIÓN 17
OBJETIVO GENERAL 18
OBJETIVOS ESPECÍFICOS 18
ANTECEDENTES 19
2. MARCO REFERENCIAL 23
2.1. Antártida 23
2.1.2. Tratado Antártico 26
2.1.3 Suelo antártico 27
2.2 Isla Decepción 29
2.3 Isla Livingston 30
2.4. Microorganismos extremófilos 31
2.5. Clasificación de los microorganismos según la temperatura 32
2.5.1 Microorganismos psicrófilos 32
2.5.2 Microorganismos termófilos 34
2.5.3 Hongos psicrófilos 35
2.6. Identificación molecular de microorganismos mediante amplificación y
secuenciación. 36
2.6.1. Extracción de ADN. 36
2.6.2 Amplificación de ADN mediante PCR 36
2.6.3 Pasos para realizar Amplificación de ADN mediante PCR 37
2.6.4. Secuenciación de productos de amplificación 38
2.6.4.1 Secuenciación mediante el método Sanger. 38
3. DISEÑO METODOLÓGICO 39
3.1 Universo, población, muestra 39
3.2 Hipótesis, Variables, indicadores 39
3.3 Técnicas y procedimientos 40
3.3.1 Tratamiento de la muestra 41
Fase 1. Muestreo 41
Fase 2. Aislamiento microbiológico en caldos 43
Fase 3. Estudio macroscópico y microscópico 46
Fase 4. Aislamiento en Medios de cultivo 47
Fase 5. Identificación BBL CRYSTAL 47
Fase 5.1 Alistamiento de las muestras para biología molecular 47
Fase 6. Biología molecular 48
4. RESULTADOS 50
4.1 Crecimiento de las muestras provenientes de la isla livingston y Decepción en
medio de cultivo líquido. 50
4.2 Obtención de cepas puras provenientes de las islas Livingston y Decepción de la
Antártida. 53
4.3 Microorganismos identificados con BBL Crystal 57
4.3 Electroforesis de productos de PCR de la región 16S rRNA a partir del ADN
extraído de cepas aisladas de muestras provenientes de las Islas Livingston y
Decepción en la Antártida. Los productos de PCR presentan el tamaño
esperado,1500pb-1800pb. 58
4.4 Resultado de la secuenciación de los productos de amplificación de PCR . 59
4.3 Crecimiento en medios de cultivo (aislamiento fúngico). 62
DISCUSIÓN 64
7. REFERENCIAS BIBLIOGRÁFICAS 72
ANEXOS 85 | spa |
dc.format.extent | 106p. | spa |
dc.format.mimetype | application/pdf | spa |
dc.language.iso | spa | spa |
dc.publisher | Universidad Colegio Mayor de Cundinamarca | spa |
dc.rights | Derechos Reservados - Universidad Colegio Mayor de Cundinamarca, 2018 | spa |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-sa/4.0/ | spa |
dc.title | Estudio de la Microbiota cultivable de suelos en dos zonas de la Antártida | spa |
dc.type | Trabajo de grado - Pregrado | spa |
dc.description.degreelevel | Pregrado | spa |
dc.description.degreename | Bacteriólogo(a) y Laboratorista Clínico | spa |
dc.identifier.barcode | 58660 | |
dc.publisher.faculty | Facultad de Ciencias de la Salud | spa |
dc.publisher.place | Bogotá D.C | spa |
dc.publisher.program | Bacteriología y Laboratorio Clínico | spa |
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dc.rights.accessrights | info:eu-repo/semantics/closedAccess | spa |
dc.rights.creativecommons | Atribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0) | spa |
dc.subject.lemb | Diversidad bacteriana | |
dc.subject.proposal | Antártida | spa |
dc.subject.proposal | Suelo | spa |
dc.subject.proposal | Isla Livingston | spa |
dc.subject.proposal | Isla Deception | spa |
dc.subject.proposal | Cultivo microbiológico | spa |
dc.subject.proposal | Microorganismos bacterianos | spa |
dc.subject.proposal | Microorganismo fúngico | spa |
dc.subject.proposal | Secuenciación de productos de PCR | spa |
dc.type.coar | http://purl.org/coar/resource_type/c_7a1f | spa |
dc.type.coarversion | http://purl.org/coar/version/c_970fb48d4fbd8a85 | spa |
dc.type.content | Text | spa |
dc.type.driver | info:eu-repo/semantics/bachelorThesis | spa |
dc.type.redcol | https://purl.org/redcol/resource_type/TP | spa |
dc.type.version | info:eu-repo/semantics/publishedVersion | spa |
dc.rights.coar | http://purl.org/coar/access_right/c_14cb | spa |