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Perfil transcripcional de genes que codifican proteínas de invasión a eritrocito pertenecientes a las familias de ligandos pfeba y pfrh de la cepa fcb2 de plasmodium falciparum

dc.contributor.advisorArarat Sarría, Mónica
dc.contributor.advisorRodríguez Panduro, Mauricio Humberto
dc.contributor.authorFlórez Arenas, Zulma Julieth
dc.date.accessioned2021-06-30T14:05:16Z
dc.date.available2021-06-30T14:05:16Z
dc.date.issued2019-10
dc.identifier.urihttps://repositorio.unicolmayor.edu.co/handle/unicolmayor/306
dc.description.abstractPlasmodium falciparum, es el parásito causante de malaria asociado a la mayor morbilidad y mortalidad a nivel mundial. Es un problema de salud pública cada vez más difícil de tratar, debido especialmente al aumento de resistencia por parte del parásito a los antimaláricos actuales; por esta razón es necesaria la búsqueda de nuevas herramientas en pro del control y la erradicación de la enfermedad principalmente enfocadas al desarrollo de vacunas eficaces. Para esto es indispensable la caracterización de proteínas que puedan ser blancos potenciales, como lo son los ligandos de invasión, correspondientes a las familias de antígenos de unión a eritrocito (PfEBAs) y proteínas homólogas de unión a reticulocito (PfRHs), ya que múltiples estudios de expresión transcripcional de los genes que codifican para estos ligandos, han demostrado que son esenciales en el proceso de invasión al eritrocito y que su expresión varía entre diferentes cepas y aislados. Por este motivo, el presente proyecto determinó de manera parcial el perfil transcripcional de genes pertenecientes a estas dos familias, cuantificando su expresión transcripcional mediante la técnica RT-qPCR, en una cepa autóctona de Colombia. Con los resultados obtenidos, se espera dar pie a futuros estudios comparativos con otras cepas del parásito, para así mejorar la comprensión de los fenotipos que utiliza P. falciparum en el proceso de invasión al eritrocito y así mismo buscar métodos para bloquear dicha invasión.spa
dc.description.tableofcontentsResumen 13 1. Introducción 14 2. Objetivos 16 2.1. Objetivo general 16 2.2. Objetivos específicos 16 3. Antecedentes 17 4. Marco referencial 22 4.1. Generalidades 22 4.2. Sintomatología y clasificación de la malaria 22 4.3. Epidemiología 24 4.4. Ciclo biológico del parásito 25 4.4.4. Vías alternas de invasión 28 4.5. Métodos de determinación del fenotipo de invasión32 4.5.1. RT-qPCR 32 5. Materiales y metodos 36 5.1. Tipo de estudio 36 5.3. Variables 36 5.4. Cultivo de parásitos 36 5.5. Extracción de ARN, ADNg y síntesis de ADNc 37 5.6. Estandarización de la técnica RT- qPCR 38 6. Resultados 41 6.1. Cuantificación de las muestras de ADN y ARN 41 6.2. Electroforesis de los genes de los genes pertenecientes a las familias PfRh y PfEBA 42 6.3. PCR tiempo real 43 6.4. Determinación parcial del perfil transcripcional de cada gen 47 7. Discusión 49 Conclusiones 53 Anexos 54 Referencias 56spa
dc.format.extent65p.spa
dc.format.mimetypeapplication/pdfspa
dc.language.isospaspa
dc.publisherUniversidad Colegio Mayor de Cundinamarcaspa
dc.relation.ispartofNo objeto asociado
dc.rightsDerechos Reservados -Universidad Colegio Myor de Cundinamarca ,2019eng
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0/spa
dc.titlePerfil transcripcional de genes que codifican proteínas de invasión a eritrocito pertenecientes a las familias de ligandos pfeba y pfrh de la cepa fcb2 de plasmodium falciparumspa
dc.typeTrabajo de grado - Pregradospa
dc.contributor.corporatenameUniversidad Colegio Mayor de Cundinamarcaspa
dc.contributor.researchgroupTrabajo de investigaciónspa
dc.coverage.countryColombia
dc.description.degreelevelPregradospa
dc.description.degreenameBacteriólogo(a) y Laboratorista Clínicospa
dc.description.researchareaTrabajo de investigaciónspa
dc.identifier.barcode60185
dc.publisher.facultyFacultad de Ciencias de la Saludspa
dc.publisher.placeBogotá, Distrito Capitalspa
dc.publisher.programBacteriología y Laboratorio Clínicospa
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dc.rights.accessrightsinfo:eu-repo/semantics/openAccessspa
dc.rights.creativecommonsAtribución-NoComercial-CompartirIgual 4.0 Internacional (CC BY-NC-SA 4.0)spa
dc.subject.lembMalaria - Epidemiología
dc.subject.lembVacuna contra la malaria
dc.subject.lembPaludismo
dc.subject.proposalMalariaspa
dc.subject.proposalPlasmodium falciparumspa
dc.subject.proposalLigandos de invasiónspa
dc.subject.proposalPfRHsspa
dc.subject.proposalPfEBAsspa
dc.subject.proposalRT-qPCRspa
dc.type.coarhttp://purl.org/coar/resource_type/c_7a1fspa
dc.type.coarversionhttp://purl.org/coar/version/c_970fb48d4fbd8a85spa
dc.type.contentTextspa
dc.type.driverinfo:eu-repo/semantics/bachelorThesisspa
dc.type.redcolhttps://purl.org/redcol/resource_type/TPspa
dc.type.versioninfo:eu-repo/semantics/publishedVersionspa
dc.rights.coarhttp://purl.org/coar/access_right/c_abf2spa


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